Article
[A method of haplotype analysis for multiple single-nucleotide polymorphisms].
Yi chuan xue bao = Acta genetica Sinica - 1 Mar 2005
Su Zhi-Guang, Zhang Si-Zhong, Xiao Cui-Ying, Tong Yu
Abstract excerpt
Haplotypes from multiple single nucleotide polymorphisms(SNPs) spaced in longer DNA were constructed by multi-step PCR and DNA sequencing methods. Two allele-specific primers were synthesized and used for long DNA fragments (approximately 10 kb) amplification from human genome DNA. Fragments within these long DNA fragments were amplified by using these PCR products as templates in the second round PCR. The second...
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