Article
Strep-tag II affinity purification: an approach to study intermediates of metalloenzyme biosynthesis.
Analytical biochemistry - 15 May 1998
Maier T, Drapal N, Thanbichler M, Böck A
Abstract excerpt
Complex metalloenzymes (e.g., nitrogenase, hydrogenase, urease) are synthesized starting from the apoprotein via several intermediates by the action of accessory proteins. The isolation and biochemical characterization of such intermediates is hampered by their low abundance and their lability. Here we describe a technique for efficient single-step purification of a hydrogenase precursor under mild conditions...
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