Article
Radioligand-dependent discrepancy in agonist affinities enhanced by mutations in the kappa-opioid receptor.
Molecular pharmacology - 1 Oct 1996
Hjorth S A, Thirstrup K, Schwartz T W
Abstract excerpt
A series of kappa/mu receptor chimeras and a number of kappa receptors substituted in the second transmembrane segment (TM-II) were investigated using as radioligands, respectively, the kappa-selective agonist [3H]C1977 and the nonselective opioid antagonist [3H]diprenorphine (DIP). All of the re...
Topics
- Animals
- Benzofurans
- COS Cells
- Diprenorphine
- Dynorphins
- Kinetics
- Mutation
- Narcotic Antagonists
- Peptide Fragments
- Pyrrolidines
- Radioligand Assay
- Receptors, Opioid, kappa
- Receptors, Opioid, mu
- Recombinant Fusion Proteins
- Tritium