Article
Antisense RNA inhibition of the putative vacuolar H(+)-ATPase proteolipid of Dictyostelium reduces intracellular Ca2+ transport and cell viability.
Journal of cell science - 1 Feb 1996
Xie Y, Coukell M B, Gombos Z
Abstract excerpt
Transport of Ca2+ via a P-type pump into the contractile vacuole of Dictyostelium discoideum appears to be facilitated by vacuolar proton (V-H+) ATPase activity. To investigate the involvement of the V-H(+)-ATPase in this process using molecular techniques, we cloned a cDNA (vatP) encoding the pu...
Topics
- Amino Acid Sequence
- Animals
- Base Sequence
- Calcium
- Cell Survival
- Cloning, Molecular
- DNA Primers
- DNA, Complementary
- Dictyostelium
- Gene Expression Regulation
- Molecular Sequence Data
- Phenotype
- Proteolipids
- Proton-Translocating ATPases
- RNA, Antisense
- Sequence Homology, Amino Acid
- Vacuoles