An assay for X inactivation based on differential methylation at the fragile X locus, FMR1.

doi:10.1002/(SICI)1096-8628(19960712)64:1<27::AID-AJMG3>3.0.CO;2-O

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Article

An assay for X inactivation based on differential methylation at the fragile X locus, FMR1.

American journal of medical genetics - 12 Jul 1996

Carrel L, Willard H F

Abstract excerpt

We describe an assay analyzing methylation at the fragile X mental retardation gene, FMR1, to examine patterns of random or non-random X chromosome inactivation. Digestion of genomic DNA with the methylation-sensitive enzyme HpaII cleaves two restriction sites near the CGG repeat of the FMR1 gene...

Topics

Alleles
Animals
Cell Line
DNA Methylation
Dosage Compensation, Genetic
Female
Fragile X Mental Retardation Protein
Fragile X Syndrome
Humans
Hybrid Cells
Male
Mice
Nerve Tissue Proteins
RNA-Binding Proteins

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