Article
Effects of NAD+ binding on the luminescence of tryptophans 84 and 310 of glyceraldehyde-3-phosphate dehydrogenase from Bacillus stearothermophilus.
Biochemistry - 24 Sept 1996
Gabellieri E, Rahuel-Clermont S, Branlant G, Strambini G B
Abstract excerpt
The individual fluorescence and phosphorescence properties of W84 and W310 in Bacillus stearothermophilus glyceraldehyde-3-phosphate dehydrogenase were identified through the construction of a single tryptophan mutant (W84F) and by comparison of the emission between mutant and wild-type enzymes....
Topics
- Adenosine Diphosphate
- Adenosine Monophosphate
- Fluorescence
- Geobacillus stearothermophilus
- Glyceraldehyde-3-Phosphate Dehydrogenases
- Kinetics
- Luminescent Measurements
- Mutagenesis, Site-Directed
- Mutation
- NAD
- Protein Conformation
- Tryptophan