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Article

Temperature-Jump Fluorescence Provides Evidence for Fully Reversible Microsecond Dynamics in a Thermophilic Alcohol Dehydrogenase.

Journal of the American Chemical Society - 19 Aug 2015

Meadows Corey W, Balakrishnan Gurusamy, Kier Brandon L, Spiro Thomas G, Klinman Judith P

Abstract excerpt

Protein dynamics on the microsecond (μs) time scale were investigated by temperature-jump fluorescence spectroscopy as a function of temperature in two variants of a thermophilic alcohol dehydrogenase: W87F and W87F:H43A. Both mutants exhibit a fast, temperature-independent μs decrease in fluorescence followed by a slower full recovery of the initial fluorescence. The results, which rule out an ionizing histidine...

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Identifiers

PMID: 26223665
DOI: 10.1021/jacs.5b04413
PMCID: PMC4970856

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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