Structure of the acid state of Escherichia coli ribonuclease HI.

doi:10.1021/bi9611671

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Article

Structure of the acid state of Escherichia coli ribonuclease HI.

Biochemistry - 17 Sept 1996

Dabora J M, Pelton J G, Marqusee S

Abstract excerpt

Under acidic conditions Escherichia coli ribonuclease HI* (RNase H*) adopts a partially folded state with all of the properties of a molten globule. Using amide hydrogen exchange carried out under acid state conditions, followed by quenching and NMR detection on the native state, we have determin...

Topics

Amino Acid Sequence
Circular Dichroism
Escherichia coli
Genetic Variation
Hydrogen-Ion Concentration
Kinetics
Magnetic Resonance Spectroscopy
Models, Molecular
Plasmids
Protein Structure, Secondary
Recombinant Proteins
Ribonuclease H

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