Reconstitution of Escherichia coli RNase HI from the N-fragment with high helicity and the C-fragment with a disordered structure.

Abstract excerpt

The Escherichia coli RNase HI variant with the Lys86-->Ala mutation is purified in two forms, as nicked and intact proteins. The nicked K86A protein, in which the N-fragment (Met1-Lys87) and the C-fragment (Arg88-Val155) remain associated, is enzymatically active. These N- and C-fragments were isolated and examined for reassociation. These peptides did not associate to form the nicked K86A protein at pH 3.0 in...

Topics

• Binding Sites
• Circular Dichroism
• Escherichia coli
• Genetic Variation
• Hydrogen-Ion Concentration
• Models, Molecular
• Molecular Structure
• Peptide Fragments
• Point Mutation
• Protein Conformation
• Protein Denaturation