Article
Genotype-dependent sex differentiation of dopaminergic neurons in primary cultures of embryonic mouse brain.
Brain research. Developmental brain research - 31 May 1996
Sibug R, Küppers E, Beyer C, Maxson S C, Pilgrim C, Reisert I
Abstract excerpt
In order to investigate genetic factors that interfere with hormone-mediated sex differentiation of dopaminergic neurons, we raised sex-specific primary cultures from embryonic day 13 diencephalon (D) or mesencephalon (M) of three different strains of mice, NMRI, CBA/J, and BALBc/J. Part of the cultures were maintained for 6 or 13 days in vitro (DIV) in medium containing 17 beta-estradiol or testosterone. The...
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