Article
A new MALDI-TOF based mini-sequencing assay for genotyping of SNPS.
Nucleic acids research - 15 Jun 2000
Sun X, Ding H, Hung K, Guo B
Abstract excerpt
A new MALDI-TOF based mini-sequencing assay termed VSET was developed for genotyping of SNPs. In this assay, specific fragments of genomic DNA containing the SNP site(s) are first amplified, followed by mini-sequencing in the presence of three ddNTPs and the fourth nucleotide in the deoxy form. In this way, the primer is extended by only one base from one allele, while it is typically extended by two bases from...
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