Characteristics of transposon insertion mutants of mandelic acid-utilizing Pseudomonas putida strain A10L.

doi:10.1271/bbb.60.1051

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Article

Characteristics of transposon insertion mutants of mandelic acid-utilizing Pseudomonas putida strain A10L.

Bioscience, biotechnology, and biochemistry - 1 Jul 1996

Shimao M, Nakamura T, Okuda A, Harayama S

Abstract excerpt

A soil isolate, Pseudomonas putida strain A10L that utilizes mandelate via the mandelate pathway was mutagenized by transposon Tn5-Mob insertion and mutant 168 lacking mandelate racemase (MR) and a mutant 254 lacking benzoylformate decarboxylase (BFDC) were obtained. Expression of (S)-mandelate d...

Topics

Aldehyde Oxidoreductases
Benzaldehyde Dehydrogenase (NADP+)
Carboxy-Lyases
DNA Transposable Elements
Mandelic Acids
Membranes
Mutagenesis
Mutation
NAD
Plasmids
Pseudomonas putida
Racemases and Epimerases
Restriction Mapping
Stereoisomerism

Source

Open in PubMed

Identifiers

PMID: 8782397
DOI: 10.1271/bbb.60.1051

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-8782397-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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