Article
Characteristics of transposon insertion mutants of mandelic acid-utilizing Pseudomonas putida strain A10L.
Bioscience, biotechnology, and biochemistry - 1 Jul 1996
Shimao M, Nakamura T, Okuda A, Harayama S
Abstract excerpt
A soil isolate, Pseudomonas putida strain A10L that utilizes mandelate via the mandelate pathway was mutagenized by transposon Tn5-Mob insertion and mutant 168 lacking mandelate racemase (MR) and a mutant 254 lacking benzoylformate decarboxylase (BFDC) were obtained. Expression of (S)-mandelate dehydrogenase (MDH), BFDC, NAD(+)-dependent benzaldehyde dehydrogenase (BDH) and NADP(+)-dependent BDH in the MR-lacking...
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