Article
Characterization of a Rhizobium meliloti proline dehydrogenase mutant altered in nodulation efficiency and competitiveness on alfalfa roots.
Molecular plant-microbe interactions : MPMI - 1 Jan 2000
Jiménez-Zurdo J I, van Dillewijn P, Soto M J, de Felipe M R, Olivares J, Toro N
Abstract excerpt
Rhizobium meliloti strain GRM8 is able to transform ornithine into proline by means of an ornithine cyclodeaminase and, therefore, has the ability to use either of these amino acids as its sole carbon and nitrogen source. By Tn5 insertion mutagenesis we obtained a GRM8 mutant derivative strain (LM1) unable to catabolize either ornithine or proline. DNA hybridization studies showed that the LM1 mutant carries a...
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