Article
Analysis of the mRNA cap-binding ability of human eukaryotic initiation factor-4E by use of recombinant wild-type and mutant forms.
European journal of biochemistry - 1 Aug 1996
Morino S, Hazama H, Ozaki M, Teraoka Y, Shibata S, Doi M, Ueda H, Ishida T, Uesugi S
Abstract excerpt
In order to identify the amino acid residues necessary for the selective recognition of the mRNA cap structure by human eukaryotic initiation factor-4E (eIF-4E), which plays a central role in the first step of mRNA translation, we prepared recombinant wild-type and fourteen mutant forms and compared their cap-binding abilities by affinity chromatography. By the direct expression of a synthetic gene encoding human...
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