Article
Analysis of the mRNA cap-binding ability of human eukaryotic initiation factor-4E by use of recombinant wild-type and mutant forms.
European journal of biochemistry - 1 Aug 1996
Morino S, Hazama H, Ozaki M, Teraoka Y, Shibata S, Doi M, Ueda H, Ishida T, Uesugi S
Abstract excerpt
In order to identify the amino acid residues necessary for the selective recognition of the mRNA cap structure by human eukaryotic initiation factor-4E (eIF-4E), which plays a central role in the first step of mRNA translation, we prepared recombinant wild-type and fourteen mutant forms and compa...
Topics
- Amino Acid Sequence
- Base Sequence
- Escherichia coli
- Eukaryotic Initiation Factor-4E
- Genes, Synthetic
- Humans
- Hydrogen-Ion Concentration
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Peptide Initiation Factors
- Protein Binding
- RNA Cap Analogs
- RNA Caps