Determination of active single chain antibody concentrations in crude periplasmic fractions.

Abstract excerpt

We have measured active single chain antibody (scFv) concentrations under mass transfer limitation conditions using surface plasmon resonance on the BIAcore. For the creation of a standard curve scFv 4Dwt, derived from monoclonal antibody (mAb) 4D, directed against human chorionic gonadotropin (hCG), was purified by positive affinity chromatography. Determination of the active antibody fraction after purification...

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