Denaturing gradient gel electrophoresis: a novel method for determining Rh phenotype from genomic DNA.

doi:10.1046/j.1365-2141.1996.d01-1808.x

Back to search

Article

Denaturing gradient gel electrophoresis: a novel method for determining Rh phenotype from genomic DNA.

British journal of haematology - 1 Aug 1996

Steers F, Wallace M, Johnson P, Carritt B, Daniels G

Abstract excerpt

Denaturing gradient gel electrophoresis (DGGE) was carried out on PCR products amplified from exons 2 and 5 of RHD and RHCE. Exon 2 of RHD and exon 2 of the C allele of RHCE have an identical sequence, which differs from that of the c allele of RHCE. One band representing D and/or C, and another...

Topics

Base Sequence
DNA
DNA Mutational Analysis
Electrophoresis
Exons
Humans
Molecular Sequence Data
Mutation
Nucleic Acid Heteroduplexes
Phenotype
Polymerase Chain Reaction
Rh-Hr Blood-Group System

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

discussions

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.