Article
Denaturing gradient gel electrophoresis: a novel method for determining Rh phenotype from genomic DNA.
British journal of haematology - 1 Aug 1996
Steers F, Wallace M, Johnson P, Carritt B, Daniels G
Abstract excerpt
Denaturing gradient gel electrophoresis (DGGE) was carried out on PCR products amplified from exons 2 and 5 of RHD and RHCE. Exon 2 of RHD and exon 2 of the C allele of RHCE have an identical sequence, which differs from that of the c allele of RHCE. One band representing D and/or C, and another representing c, could be distinguished by DGGE of exon 2 amplifications of genomic DNA from individuals with the...
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