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Single-step purifications of His6-MutH, His6-MutL and His6-MutS repair proteins of escherichia coli K-12.

BioTechniques - 1 Dec 1995

Feng G, Winkler M E

Abstract excerpt

The MutS, MutH and MutL proteins mediate methyl-directed-mismatch (MDM) repair in Escherichia coli and Salmonella typhimurium. These proteins have been developed into powerful tools for screening genomes for polymorphisms and detecting and localizing mutations. In an ongoing study of the regulation of MDM repair, we developed one-step schemes to purify the E. coli MutS, MutH and MutL proteins fused to a...

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PMID: 8747662

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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Single-step purifications of His6-MutH, His6-MutL and His6-MutS repair proteins of escherichia coli K-12.PMID 8747662Read ↗

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