Article
The use of antigen retrieval for immunohistochemical detection of p53 over-expression in malignant and benign oral mucosa: a cautionary note.
Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology - 1 Feb 1996
Dowell S P, Ogden G R
Abstract excerpt
The tumour suppressor gene p53 is frequently mutated in neoplasia. Since mutant p53 protein is often over-expressed, mutation can be indirectly detected by immunocytochemical techniques. As microwave antigen retrieval is becoming a widespread method for increasing the antigenicity of paraffin sec...
Topics
- Antigens, Neoplasm
- Carcinoma, Squamous Cell
- Coloring Agents
- Gene Expression Regulation, Neoplastic
- Genes, p53
- Humans
- Immunohistochemistry
- Microwaves
- Mouth Diseases
- Mouth Mucosa
- Mouth Neoplasms