Article
Catalytic activities of alpha3beta3gamma complexes of F1-ATPase with 1, 2, or 3 incompetent catalytic sites.
The Journal of biological chemistry - 26 Jul 1996
Amano T, Hisabori T, Muneyuki E, Yoshida M
Abstract excerpt
In order to know how many functional catalytic sites are necessary for ATPase activity of F1-ATPase from a thermophilic Bacillus PS3, a new method of isolating homogeneous preparations of the alpha3beta3gamma complex with 1, 2, or 3 incompetent catalytic sites was developed. Ten glutamic acids (G...
Topics
- Adenosine Diphosphate
- Adenosine Triphosphate
- Bacillus
- Base Sequence
- Binding Sites
- Catalysis
- Hydrolysis
- Molecular Sequence Data
- Mutation
- Protein Conformation
- Proton-Translocating ATPases
- Spectrophotometry