Expression of the wild-type and the Cys-/Trp-less alpha 3 beta 3 gamma complex of thermophilic F1-ATPase in Escherichia coli.

doi:10.1016/0005-2728(95)00070-y

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Article

Expression of the wild-type and the Cys-/Trp-less alpha 3 beta 3 gamma complex of thermophilic F1-ATPase in Escherichia coli.

Biochimica et biophysica acta - 12 Sept 1995

Matsui T, Yoshida M

Abstract excerpt

The alpha, beta and gamma subunits of F1-ATPase from thermophilic Bacillus PS3 were expressed in Escherichia coli cells simultaneously in large amounts. Most of the expressed subunits assembled into a form of alpha 3 beta 3 gamma complex in E. coli cells and this complex was easily purified to ho...

Topics

Bacillus
Base Sequence
Escherichia coli
Gene Expression
Kinetics
Molecular Sequence Data
Mutation
Plasmids
Proton-Translocating ATPases
Recombinant Proteins
Temperature

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