Article
Complete inhibition and partial Re-activation of single F1-ATPase molecules by tentoxin: new properties of the re-activated enzyme.
The Journal of biological chemistry - 12 Mar 2004
Pavlova Penka, Shimabukuro Katsuya, Hisabori Toru, Groth Georg, Lill Holger, Bald Dirk
Abstract excerpt
During hydrolysis of ATP, the gamma subunit of the rotary motor protein F(1)-ATPase rotates within a ring of alpha(3)beta(3) subunits. Tentoxin is a phyto-pathogenic cyclic tetrapeptide, which influences F(1)-ATPase activity of sensitive species. At low concentrations, tentoxin inhibits ATP hydrolysis of ensembles of F(1) molecules in solution. At higher concentrations, however, ATP hydrolysis recovers. Here we...
Topics
- ATP Synthetase Complexes
- Adenosine Diphosphate
- Adenosine Triphosphate
- Bacillus
- Binding Sites
- Chloroplast Proton-Translocating ATPases
- Dose-Response Relationship, Drug
- Escherichia coli
- Hydrolysis
- Kinetics
- Mutation
- Peptides, Cyclic
- Protein Binding