Article
The alpha 3 beta 3 gamma complex of the F1-ATPase from thermophilic Bacillus PS3 containing the alpha D261N substitution fails to dissociate inhibitory MgADP from a catalytic site when ATP binds to noncatalytic sites.
Biochemistry - 19 Dec 1995
Jault J M, Matsui T, Jault F M, Kaibara C, Muneyuki E, Yoshida M, Kagawa Y, Allison W S
Abstract excerpt
ATP hydrolyses by the wild-type alpha 3 beta 3 gamma and mutant (alpha D261N)3 beta 3 gamma subcomplexes of the F1-ATPase from the thermophilic Bacillus PS3 have been compared. The wild-type complex hydrolyzes 50 microM ATP in three kinetic phases: a burst decelerates to an intermediate phase, which then gradually accelerates to a final rate. In contrast, the mutant complex hydrolyzes 50 microM or 2 mM ATP in two...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.