Article
Real-time monitoring of conformational dynamics of the epsilon subunit in F1-ATPase.
The Journal of biological chemistry - 2 Dec 2005
Iino Ryota, Murakami Tomoe, Iizuka Satoshi, Kato-Yamada Yasuyuki, Suzuki Toshiharu, Yoshida Masasuke
Abstract excerpt
It has been proposed that C-terminal two alpha-helices of the epsilon subunit of F1-ATPase can undergo conformational transition between retracted folded-hairpin form and extended form. Here, using F(1) from thermophilic Bacillus PS3, we monitored this transition in real time by fluorescence resonance energy transfer (FRET) between a donor dye and an acceptor dye attached to N terminus of the gamma subunit and C...
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