Article
X-ray structure of human beta3beta3 alcohol dehydrogenase. The contribution of ionic interactions to coenzyme binding.
The Journal of biological chemistry - 19 Jul 1996
Davis G J, Bosron W F, Stone C L, Owusu-Dekyi K, Hurley T D
Abstract excerpt
The three-dimensional structure of the human beta3beta3 dimeric alcohol dehydrogenase (beta3) was determined to 2.4-A resolution. beta3 was crystallized as a ternary complex with the coenzyme NAD+ and the competitive inhibitor 4-iodopyrazole. beta3 is a polymorphic variant at ADH2 that differs fr...
Topics
- Alcohol Dehydrogenase
- Amino Acid Sequence
- Arginine
- Base Sequence
- Binding Sites
- Calorimetry
- Crystallography, X-Ray
- Cysteine
- DNA, Complementary
- Genetic Variation
- Humans
- Isoenzymes
- Macromolecular Substances
- Models, Molecular
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- NAD
- Oligodeoxyribonucleotides