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Article

Contributions of active site residues to cofactor binding and catalysis of 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase.

Biochimica et biophysica acta - 1 Jan 2010

Chang Yi-Hsun, Wang Chau-Zen, Chiu Chien-Chih, Chuang Lea-Yea, Hwang Chi-Ching

Abstract excerpt

3alpha-Hydroxysteroid dehydrogenase/carbonyl reductase reversely catalyzes the oxidation of androsterone with NAD(+) to form androstanedione and NADH. In this study, we investigated the function of active site residues N86, Y155, and K159 in NADH binding and catalysis in the reduction of androsta...

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Identifiers

PMID: 19853671
DOI: 10.1016/j.bbapap.2009.10.012

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 2 Sept 2026. Prepared record publication-19853671-2026-09-02.2. Manifest publication-graph-cohort-2026-09-02.2.

Source receipt: PubMed via Helena Literature Corpus, retrieved 2 Sept 2026.

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