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Article

Exploring the active site of chorismate mutase by combinatorial mutagenesis and selection: the importance of electrostatic catalysis.

Proceedings of the National Academy of Sciences of the United States of America - 14 May 1996

Kast P, Asif-Ullah M, Jiang N, Hilvert D

Abstract excerpt

Chorismate mutase (EC 5.4.99.5) catalyzes the intramolecular rearrangement of chorismate to prephenate. Arg-90 in the active site of the enzyme from Bacillus subtilis is in close proximity to the substrate's ether oxygen and may contribute to efficient catalysis by stabilizing the presumed dipolar transition state that would result upon scission of the C--O bond. To test this idea, we have developed a novel...

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Identifiers

PMID: 8643526
DOI: 10.1073/pnas.93.10.5043
PMCID: PMC39403

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