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Article

Complementation analysis of mutants of nitric oxide synthase reveals that the active site requires two hemes.

Proceedings of the National Academy of Sciences of the United States of America - 14 May 1996

Xie Q W, Leung M, Fuortes M, Sassa S, Nathan C

Abstract excerpt

For catalytic activity, nitric oxide synthases (NOSs) must be dimeric. Previous work revealed that the requirements for stable dimerization included binding of tetrahydrobiopterin (BH4), arginine, and heme. Here we asked what function is served by dimerization. We assessed the ability of individually inactive mutants of mouse inducible NOS (iNOS; NOS2), each deficient in binding a particular cofactor or...

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Identifiers

PMID: 8643499
DOI: 10.1073/pnas.93.10.4891
PMCID: PMC39375

Genes

NOS2

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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