Article
Stability and folding of precursor and mature tryptophan-substituted ribose binding protein of Escherichia coli.
Archives of biochemistry and biophysics - 1 Apr 1996
Lee H, Chi S W, Kang M, Baek K, Kim H
Abstract excerpt
A mutant ribose binding protein (RBP) of Escherichia coli was obtained by site-directed mutagenesis, replacing Phe-187 in the wild-type RBP (WT-RBP) with a Trp residue, in order to compare its stability and folding behavior with those of the WT-RBP. The equilibrium unfolding properties and the folding kinetics of these proteins were monitored by fluorescence and circular dichroism (CD). For both WT-RBP and the...
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