Article
Cooperative unfolding of Escherichia coli ribosome recycling factor originating from its domain-domain interaction and its implication for function.
Archives of biochemistry and biophysics - 15 Jun 2006
Zhang Liqiang, Guo Peng, Zhang Hongjie, Jing Guozhong
Abstract excerpt
Cooperative unfolding of Escherichia coli ribosome recycling factor (RRF) and its implication for function were investigated by comparing the in vitro unfolding and the in vivo activity of wild-type E. coli RRF and its temperature-sensitive mutant RRF(V117D). The experiments show that mutation V117D at domain I could perturb the domain II structure as evidenced in the near-UV CD and tyrosine fluorescence spectra...
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