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Article

Astrovirus ribosomal frameshifting in an infection-transfection transient expression system

1 May 1996

Abstract excerpt

Different regions of the human astrovirus frameshift signal were cloned into the rhesus rotavirus VP4 gene and evaluated in an infection-transfection transient expression cell culture system. BHK-21 cells, infected with a vaccinia virus that expresses T7 RNA polymerase (vTF7-3), were transfected with the various astrovirus-VP4 constructs. All constructs were driven by a T7 promoter and contained an internal...

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PMID: 8627761
DOI: 10.1128/jvi.70.5.2869-2875.1996

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