Article
Generation of astaxanthin mutants in Xanthophyllomyces dendrorhous using a double recombination method based on hygromycin resistance.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2012
Niklitschek Mauricio, Baeza Marcelo, Fernández-Lobato María, Cifuentes Víctor
Abstract excerpt
Generally two selection markers are required to obtain homozygous mutations in a diploid background, one for each gene copy that is interrupted. In this chapter is described a method that allows the double gene deletions of the two copies of a gene from a diploid organism, a wild-type strain of the Xanthophyllomyces dendrorhous yeast, using hygromycin B resistance as the only selection marker. To accomplish this,...
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