Article
Cloning and characterization of the eapB and eapC genes of Cryphonectria parasitica encoding two new acid proteinases, and disruption of eapC.
Molecular & general genetics : MGG - 15 Jan 1996
Jara P, Gilbert S, Delmas P, Guillemot J C, Kaghad M, Ferrara P, Loison G
Abstract excerpt
Two new proteinases secreted by Cryphonectria parasitica, namely EapB and EapC, have been purified. The corresponding structural genes were isolated by screening a cosmid library, and sequenced. Comparison of genomic and cDNA sequences revealed that the eapB and eapC genes contain three and two i...
Topics
- Amino Acid Sequence
- Ascomycota
- Aspartic Acid Endopeptidases
- Base Sequence
- Cloning, Molecular
- DNA, Fungal
- Endopeptidases
- Enzyme Precursors
- Fungal Proteins
- Genes, Fungal
- Molecular Sequence Data
- Mutation
- Sequence Homology, Amino Acid