Article
Cloning and targeted disruption of enpg-1, encoding the major in vitro extracellular endopolygalacturonase of the chestnut blight fungus, Cryphonectria parasitica.
Applied and environmental microbiology - 1 Jun 1996
Gao S, Choi G H, Shain L, Nuss D L
Abstract excerpt
The gene enpg-1, encoding the major extracellular endopolygalacturonase (endoPG) purified from culture filtrates of the chestnut blight fungus, Cryphonectria parasitica, was cloned and characterized. The deduced mature enpg-1 protein product, ENPG-1, had a calculated molecular mass of 34.5 kDa an...
Topics
- Amino Acid Sequence
- Ascomycota
- Base Sequence
- Chromosome Mapping
- Cloning, Molecular
- DNA Primers
- DNA, Fungal
- Extracellular Space
- Gene Targeting
- Genes, Fungal
- Isoelectric Point
- Molecular Sequence Data
- Molecular Weight
- Mutation
- Phenotype
- Plant Diseases
- Polygalacturonase
- Sequence Homology, Amino Acid