Article
Cloning and mutation of the gene encoding endothiapepsin from Cryphonectria parasitica.
Current genetics - 1 May 1992
Razanamparany V, Jara P, Legoux R, Delmas P, Msayeh F, Kaghad M, Loison G
Abstract excerpt
Endothiapepsin is an aspartic protease secreted by Cryphonectria parasitica. It has a milk-clotting activity and is used in the cheese industry. The eapA gene encoding endothiapepsin has been cloned and sequenced. An open reading frame of 419 codons, which encodes a precursor differing from mature endothiapepsin by the presence of an 89 aa residue prepro-sequence, was found. The eapA gene is interrupted by three...
Topics
- Amino Acid Sequence
- Ascomycota
- Aspartic Acid Endopeptidases
- Base Sequence
- Blotting, Southern
- Cloning, Molecular
- Fungal Proteins
- Molecular Sequence Data
- Mutation
- Oligonucleotide Probes
- Restriction Mapping