Article
Amplification of lambda plasmids in Escherichia coli relA mutants.
Journal of biotechnology - 1 Dec 1995
Wegrzyn G
Abstract excerpt
It was previously demonstrated that, contrary to wild-type stringent (rel+) strains of Escherichia coli, in amino acid-starved relaxed (relA) mutants the replication of lambda plasmid proceeds for several hours. The replication leads to amplification of lambda plasmid DNA. Here, the conditions fo...
Topics
- Bacteriophage lambda
- Escherichia coli
- Gene Amplification
- Ligases
- Mutation
- Plasmids
