Article
Single-cell analysis of Hodgkin and Reed-Sternberg cells: molecular heterogeneity of gene expression and p53 mutations.
Blood - 1 Jun 1993
Trümper L H, Brady G, Bagg A, Gray D, Loke S L, Griesser H, Wagman R, Braziel R, Gascoyne R D, Vicini S
Abstract excerpt
We have used a single-cell based polymerase chain reaction (PCR) amplification technique to examine the gene expression pattern in single Hodgkin's and Reed-Sternberg (H&RS) cells from seven patients with Hodgkin's disease. Single cells were isolated from lymph nodes obtained at diagnosis (5 of 7...
Topics
- Adolescent
- Adult
- Base Sequence
- Cloning, Molecular
- Cytokines
- Female
- Gene Expression
- Genes, p53
- Hodgkin Disease
- Humans
- Lymph Nodes
- Male
- Middle Aged
- Molecular Sequence Data
- Mutation
- Oligodeoxyribonucleotides
- Polymerase Chain Reaction
- RNA, Messenger