Article
TAP1, a yeast gene that activates the expression of a tRNA gene with a defective internal promoter.
Molecular and cellular biology - 1 Jun 1993
Di Segni G, McConaughy B L, Shapiro R A, Aldrich T L, Hall B D
Abstract excerpt
We developed a genetic selection system based on nonsense suppression in Saccharomyces cerevisiae to identify mutations in proteins involved in transcription initiation by RNA polymerase III. A SUP4 tRNA(Tyr) internal promoter mutation (A53T61) that was unable to suppress ochre mutations in vivo...
Topics
- Alleles
- Binding, Competitive
- Cloning, Molecular
- Crosses, Genetic
- DNA, Fungal
- Exoribonucleases
- Fungal Proteins
- Genes, Fungal
- Mutation
- Plasmids
- Promoter Regions, Genetic
- RNA, Fungal
- RNA, Transfer
- RNA, Transfer, Tyr
- Restriction Mapping
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae Proteins
- Suppression, Genetic