Article
TAP1, a yeast gene that activates the expression of a tRNA gene with a defective internal promoter.
Molecular and cellular biology - 1 Jun 1993
Di Segni G, McConaughy B L, Shapiro R A, Aldrich T L, Hall B D
Abstract excerpt
We developed a genetic selection system based on nonsense suppression in Saccharomyces cerevisiae to identify mutations in proteins involved in transcription initiation by RNA polymerase III. A SUP4 tRNA(Tyr) internal promoter mutation (A53T61) that was unable to suppress ochre mutations in vivo and was incapable of binding TFIIIC in vitro was used as the target for selection of trans-acting compensatory...
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