Article
Effects of amino acid substitutions in the -10 binding region of sigma E from Bacillus subtilis.
Journal of bacteriology - 1 Nov 1992
Jones C H, Tatti K M, Moran C P
Abstract excerpt
The sigma subunit of bacterial RNA polymerase is required for specific binding to promoters. One region in most sigma factors makes sequence-specific contacts at the -10 region of its cognate promoters. To test the role of the amino acids in this -10 binding region, we examined the effects of 49 single-amino-acid substitutions in sigma E from Bacillus subtilis. We assayed the effect of each amino acid...
Topics
- Alleles
- Bacillus subtilis
- Bacterial Proteins
- Base Sequence
- Binding Sites
- DNA-Directed RNA Polymerases
- Genes, Dominant
- Genes, Recessive
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Promoter Regions, Genetic