Structural and functional properties of a Bacillus subtilis temperature-sensitive sigma(A) factor.

doi:10.1002/1097-0134(20000901)40:4<613::aid-prot60>3.0.co;2-k

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Article

Structural and functional properties of a Bacillus subtilis temperature-sensitive sigma(A) factor.

Proteins - 1 Sept 2000

Wen Y D, Liao C T, Liou K M, Wang W H, Huang W C, Chang B Y

Abstract excerpt

Bacillus subtilis DB1005 is a temperature-sensitive (Ts) sigA mutant containing double-amino-acid substitutions (I198A and I202A) on the hydrophobic face of the promoter -10 binding helix of sigma(A) factor. We have analyzed the structural and functional properties of this mutant sigma(A) factor both in vivo and in vitro. Our data revealed that the Ts sigma(A) factor possessed predominantly a multimeric structure...

Topics

Amino Acid Substitution
Bacillus subtilis
Chromatography, Gel
Electrophoresis, Polyacrylamide Gel
Mutation
Protein Binding
Protein Denaturation
Sigma Factor
Spectrometry, Fluorescence
Temperature
Transcription, Genetic

Source

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Identifiers

PMID: 10899785
DOI: 10.1002/1097-0134(20000901)40:4<613::aid-prot60>3.0.co;2-k

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