Article
A pleîotropic acid phosphatase-deficient mutant of Escherichia coli shows premature termination in the dsbA gene. Use of dsbA::phoA fusions to localize a structurally important domain in DsbA.
Molecular & general genetics : MGG - 1 Jan 1994
Belin P, Quéméneur E, Boquet P L
Abstract excerpt
A one-step mutant of Escherichia coli K-12 lacking both glucose-1-phosphatase (Agp) and pH 2.5 acid phosphatase (AppA) activities in the periplasmic space was isolated. The mutation which mapped close to chlB, at 87 min on the E. coli linkage map, also caused the loss of alkaline phosphatase (Pho...
Topics
- Acid Phosphatase
- Amino Acid Sequence
- Bacterial Proteins
- Base Sequence
- Cell Membrane
- Cloning, Molecular
- DNA Mutational Analysis
- DNA Transposable Elements
- DNA, Bacterial
- DNA, Recombinant
- Disulfides
- Escherichia coli
- Genes, Bacterial
- Molecular Sequence Data
- Mutation
- Recombinant Fusion Proteins
- Restriction Mapping
- Structure-Activity Relationship