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Detection of Aspergillus fumigatus by polymerase chain reaction

1 Mar 1993

Abstract excerpt

Aspergillus fumigatus is an opportunistic nosocomial pathogen causing an often fatal pneumonia, invasive aspergillosis (IA), in immunosuppressed patients. Oligonucleotide primers were used to amplify a 401-bp fragment spanning the 26S/intergenic spacer region of the rDNA complex of A. fumigatus by the polymerase chain reaction (PCR). The primers were highly sensitive and specific: as little as 1 pg of A....

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PMID: 8458955
DOI: 10.1128/jcm.31.3.615-621.1993

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