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Detection of a wide range of medically important fungi by the polymerase chain reaction

1 May 1994

Abstract excerpt

A polymerase chain reaction (PCR) method was developed that was capable of detecting a wide range of medically important fungi from clinical specimens. The primer pair was designed in conserved sequences of 18S-ribosomal RNA genes shared by most fungi. The lower limit of detection of this PCR technique was 1 pg of Candida albicans genomic DNA by ethidium bromide staining and 100 fg after Southern analysis. A...

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PMID: 8176723
DOI: 10.1099/00222615-40-5-358

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