PCR identification system for the genus<i>Aspergillus</i>and three major pathogenic species:<i>Aspergillus fumigatus</i>,<i>Aspergillus flavus</i>and<i>Aspergillus niger</i>

Abstract excerpt

A PCR system was developed that allowed recognition of three major pathogenic Aspergillus species, namely A. fumigatus, A. niger and A. flavus, in isolates obtained from clinical specimens. The primer pair for PCR was designed from conserved sequences of internal transcribed spacer 1 (ITS1) ribosomal DNA and its flanking regions. Products 521 bp in size were successfully amplified by PCR from the seven...

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