Article
Isolation and characterization of human and rat cardiac microvascular endothelial cells.
The American journal of physiology - 1 Feb 1993
Nishida M, Carley W W, Gerritsen M E, Ellingsen O, Kelly R A, Smith T W
Abstract excerpt
Although reciprocal intercellular signaling may occur between endocardial or microvascular endothelium and cardiac myocytes, suitable in vitro models have not been well characterized. In this report, we describe the isolation and primary culture of cardiac microvascular endothelial cells (CMEC) f...
Topics
- Animals
- Cell Division
- Cell Separation
- Coronary Circulation
- Culture Media
- Cytological Techniques
- Endothelium, Vascular
- Flow Cytometry
- Heart Ventricles
- Humans
- Immunohistochemistry
- Microcirculation
- Myocardium
- Phenotype
- Rats