Article
TAR RNA binding properties and relative transactivation activities of human immunodeficiency virus type 1 and 2 Tat proteins.
Journal of virology - 1 Feb 1993
Rhim H, Rice A P
Abstract excerpt
Using gel shift assays, we found that the human immunodeficiency virus type 1 (HIV-1) Tat protein (Tat-1) bound both HIV-1 and HIV-2 TAR RNAs with similar high affinities. In contrast, the HIV-2 Tat protein (Tat-2) bound only TAR-2 RNA with high affinity. We conclude that the weak in vivo activit...
Topics
- Amino Acid Sequence
- Binding, Competitive
- DNA Mutational Analysis
- Gene Expression Regulation, Viral
- Gene Products, tat
- HIV
- HIV-1
- HIV-2
- Molecular Sequence Data
- Mutation
- RNA, Viral
- Recombinant Fusion Proteins
- Regulatory Sequences, Nucleic Acid
- Transcriptional Activation
- tat Gene Products, Human Immunodeficiency Virus