Article
The Salmonella typhimurium nadC gene: sequence determination by use of Mud-P22 and purification of quinolinate phosphoribosyltransferase.
Journal of bacteriology - 1 Jan 1993
Hughes K T, Dessen A, Gray J P, Grubmeyer C
Abstract excerpt
The Salmonella typhimurium nadC gene and its product, quinolinic acid phosphoribosyltransferase (QAPRTase), were characterized at the molecular and biochemical levels. Fusions of Mud-lac elements isolated in the nadC gene were converted to Mud-P22 insertions. Starting with six original Mud-lac fu...
Topics
- Amino Acid Sequence
- Bacteriophage P22
- Base Sequence
- Chromatography, Gel
- Chromatography, Ion Exchange
- Cloning, Molecular
- DNA, Bacterial
- Escherichia coli
- Genes, Bacterial
- Genetic Vectors
- Genotype
- Kinetics
- Molecular Sequence Data
- Pentosyltransferases
- Plasmids