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Article

Mutational and structural analysis of cobalt-containing nitrile hydratase on substrate and metal binding.

European journal of biochemistry - 1 Jan 2004

Miyanaga Akimasa, Fushinobu Shinya, Ito Kiyoshi, Shoun Hirofumi, Wakagi Takayoshi

Abstract excerpt

Mutants of a cobalt-containing nitrile hydratase (NHase, EC 4.2.1.84) from Pseudonocardia thermophila JCM 3095 involved in substrate binding, catalysis and formation of the active center were constructed, and their characteristics and crystal structures were investigated. As expected from the str...

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Identifiers

PMID: 14717710
DOI: 10.1046/j.1432-1033.2003.03943.x

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 2 Sept 2026. Prepared record publication-14717710-2026-09-02.2. Manifest publication-graph-cohort-2026-09-02.2.

Source receipt: PubMed via Helena Literature Corpus, retrieved 2 Sept 2026.

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