Article
Problems related to the interpretation of autoradiographic data on gene expression using common constitutive transcripts as controls.
Nucleic acids research - 11 Aug 1993
Spanakis E
Abstract excerpt
The 28S rRNA, a ribosomal RNA, and the ACTB and GAPD mRNAs, coding respectively for beta-actin and glyceraldehyde-3-phosphate dehydrogenase (GAPDH), are frequently presented as controls of modulated gene expression. These transcripts were quantified by replicate slot-blot autoradiography and imag...
Topics
- Actins
- Autoradiography
- Cells, Cultured
- Data Interpretation, Statistical
- Gene Expression
- Genetic Variation
- Glyceraldehyde-3-Phosphate Dehydrogenases
- Image Processing, Computer-Assisted
- Organ Specificity
- RNA, Messenger
- RNA, Ribosomal, 28S
- Transcription, Genetic