Article
Denaturing gradient gel electrophoresis of the alpha 1-antitrypsin gene: application to prenatal diagnosis.
American journal of medical genetics - 1 Oct 1991
Dubel J R, Finwick R, Hejtmancik J F
Abstract excerpt
A method for detection of the M and Z alleles of the alpha 1-antitrypsin deficiency (AAT) gene has been developed using denaturing gradient gel electrophoresis (DGGE) of DNA samples amplified in vitro by the polymerase chain reaction. Amplification of the 90 nucleotides surrounding the Z mutation site with concurrent attachment of a 40 bp GC-rich region yields DNA fragments that are easily and quickly separated...
Topics
- Base Sequence
- Electrophoresis, Polyacrylamide Gel
- Female
- Fetal Diseases
- Humans
- Male
- Molecular Sequence Data
- Mutation
- Oligodeoxyribonucleotides
- Polymerase Chain Reaction
- Prenatal Diagnosis