Article
Pentose-phosphate pathway in Saccharomyces cerevisiae: analysis of deletion mutants for transketolase, transaldolase, and glucose 6-phosphate dehydrogenase.
Current genetics - 1 Nov 1993
Schaaff-Gerstenschläger I, Zimmermann F K
Abstract excerpt
Deletion mutants for the yeast transketolase gene TKL1 were constructed by gene replacement. Transketolase activity was below the level of detection in mutant crude extracts. Transketolase protein could be detected as a single protein band of the expected size by Western-blot analysis in wild-typ...
Topics
- Base Sequence
- Blotting, Southern
- Blotting, Western
- Cloning, Molecular
- DNA, Fungal
- Genes, Fungal
- Glucosephosphate Dehydrogenase
- Molecular Sequence Data
- Mutation
- Pentoses
- Phenotype
- Phosphates
- Saccharomyces cerevisiae
- Sequence Deletion
- Transaldolase
- Transketolase